IHC Reagents

BCIP/NBT, stable alkaline phosphatase substrate, 100 mL

Stabilized Chromogen Solution
This alkaline phosphatase chromogen solution contains the two components in a stabilized solution which may be used as the final step in color development. The resulting product is a permanent, deep-purple stain. It enhances results in both ELISA and immunohistology assays utilizing peroxidase and alkaline phosphatase.


3,3-Diaminobenzidine tablets, 100 tabs

Used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. Insoluble end product is resistant to alcohol and fading, making it ideal for immunohistochemistry.

Immuno-fluore mounting medium, 25 mL

Immuno-Fluore™ Mounting Medium is a mounting medium for immunofluorescence preparations. A stabilizing agent has been added that inhibits the fading of fluorescence under the influence of ultraviolet light.

Peroxidase protective buffer, 500 mL

Peroxidase Protective Buffer which stabilizes HRP conjugates. This protective buffer is compatible with common blocking agents such as BSA, skim milk (Blotto), Tween-20 and normal serum.

TMB (3,3',5,5' Tetramethylbenzidine)

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.


BCIP/NBT, liquid substrate plus, 100 mL

BCIP/NBT Liquid Substrate Plus is a stabilized chromogen solution. It has a greater sensitivity and produces little or no background staining compared with other alternatives. It is ready to use solution.

BCIP/NBT, liquid substrate, 100 mL

(5-Bromo-4-chloro-3-indolyl Phosphate/Nitroblue Tetrazolium)Stabilized Chromogen SolutionSpecially prepared for immunoblotting procedures only. Concentration: 0.577 mmol/liter BCIP; 0.122 mmol/liter NBT.